FIGURE 5.

PCR array analysis of indicated genes in dendritic cells that overexpress VentX. Monocytes were transfected with pcDNA-GFP or pcDNA-GFP.VentX plasmids through electroporation. GM-CSF and IL4 were added to culture medium 2 h later to induce DCs differentiation. 3 days after transfection, LPS was added for overnight treatment, and cells were harvested and sorted with GFP through flow cytometry. A–E, total RNA was isolated, and the mRNA levels of indicated genes were analyzed by PCR array. Results shown are one representative of two independent experiments. F, mRNA levels of selected genes were determined by real-time PCR. Results shown are mean ± S.D. of triplicates, and the differences are significantly different between two groups (p < 0.05) for all genes.