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Orthogonal validation of mitochondrial protein expression. Mitochondria and cytosolic fractions were prepared from SY5Y cells. Samples were separated on SDS-PAGE and probed for specific proteins. COX IV is used as a mitochondria load control whereas actin was a cytoplasmic load control. ACADM, NDUFA9, NDUFA5, and MTX1 expression mirrored the expression changes in the proteomic studies. WB, Western blotting.
