FIGURE 1.

POMGnT1 interacts and co-localizes with GOLPH3 proteins. A, HeLa cells were transfected with an expression plasmid to ectopically express GOLPH3- and HA-tagged POMGnT1. IP was performed using the cell lysates prepared from the transfected cells and an anti-HA antibody. The immunoprecipitated complexes and the protein inputs were analyzed by Western blotting (Western) using anti-GOLPH3 antibodies. GOLPH3 displayed interactions with POMGnT1. B, the cell lysates of GOLPH3 and POMGnT1-HA expressing cells were used for IP with an anti-GOLPH3 antibody. Normal IgG (IgG) was used as a negative control. The presence of POMGnT1 in the immunoprecipitates was determined by Western blotting using anti-HA antibodies. C, POMGnT1-HA was co-expressed with GOLPH3 and fixed cells were stained with anti-HA (green) and anti-GOLPH3 (red) antibodies and DAPI. GOLPH3 co-localized with POMGnT1 at the Golgi.