TABLE 1.
Primers for Nmnat3gt/gt mice genotyping and mouse Nmnat3 qPCR
Locations of the primers used in geneotyping for Nmnat3gt/gt mice are indicated in Fig. 2A. Locations of the primers used for qPCR of Nmnat3 mRNA are indicated in Fig. 2B.
| Sequence | |
|---|---|
| Genotyping primers for Nmnat3gt/gt mice | |
| Primer #1 | TCTTCTGGGGTCGCAGTTAT |
| Primer #2 | CCTTCTTTCTGGTCTTTCTCTGTGCAA |
| Primer #3 | TGCCACCTGACGTCTAAGAA |
| Primer #4 | GACAGTGCAGCGATGTCCTA |
| qPCR primers for mouse Nmnat3 | |
| Nmnat3-Ex1 | Fwd: GTGTCCACGAAGCCTTGAGT |
| Rev: CAGCCATCTGACTCTGTCGT | |
| Nmnat3-Ex2–3 | Fwd: CACCAAACAGGAAGGTACCA |
| Rev: AAGCCACCAGGTCTTTCTTC | |
| Nmnat3-Ex5 | Fwd: CAGGGTTCCCAATATCCTGA |
| Rev: TCAAACAAGCAGGCAGTCAT | |
| B2m (β2-microglobulin) | Fwd: TTCTGGTGCCTTGTCTCACTGA |
| Rev: CAGTATGTTCGGCTTCCCATTC | |
| Rpl13a (ribosomal protein L13a) | Fwd: AGCGCCTCAAGGTGTTGGA |
| Rev: GAGTGGCTGTCACTGCCTGGTA | |