FIGURE 4.

Grx2 deficiency does not induce oxidative damage in cardiac mitochondria. a, measurement of 4-hydroxy-2-nonenal (4-HNE) histidine adduct levels in isolated mitochondria. n = 4, mean ± S.E., Student's t test. b, assessment of 8-hydroxy-2′-deoxyguanosine (8-OH-2′-deoxyguanosine) levels in isolated mitochondria. n = 4, mean ± S.E., Student's t test. c, immunoblot analysis of MnSOD, uncoupling protein-3 (UCP3) protein levels, Grx2, and Trx2. Succinate dehydrogenase (SDH) or α-tubulin served as loading controls.