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TRPP2 with mutations in four N-glycosylation sites is still sensitive to enzymatic deglycosylation. TRPP2Δ4-Glyc was expressed heterologously in HeLa cells, immunoprecipitated (IP), and analyzed by SDS-PAGE and subsequent anti-TRPP2 Western blot (WB) staining. Enzymatic deglycosylation of TRPP2Δ4-Glyc with Endo H or PNGase F caused a shift in electrophoretic mobility (∼3 kDa), indicating that TRPP2Δ4-Glyc is N-glycosylated.
