FIGURE 9.

Analysis of retinaldehyde reductase and retinol dehydrogenase activities in Dhrs3^−/−versus WT mice. MEFs isolated from E14.5 embryos were incubated with 5 μm retinaldehyde (A) or 10 μm retinol (C) for 12 h. Extracted retinoids were separated by normal phase HPLC and normalized per amount of cellular protein. **, p < 0.01, mean ± S.D., n = 3. Note that the conversion of retinol (ROL) to retinaldehyde (RAL) and atRA (RA) was reduced in Dhrs3^−/− mice, supporting the in vitro data on activation of RDH10 by DHRS3. Membrane fractions (30 μg) isolated from wild-type (WT) or Dhrs3^−/− (KO) E14.5 embryos were incubated with 1 μm retinaldehyde (B) or 2 μm retinol (D). **, p < 0.01, mean ± S.D., n = 3 embryos. Note that the membrane-associated retinaldehyde reductase and retinol dehydrogenase activities are both decreased in E14.5 Dhrs3^−/− embryos.