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. 2014 Apr 11;289(21):14981–14995. doi: 10.1074/jbc.M113.529354

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Histone H3 association with active gene promoters is increased in Mediator mutants. A, histone H3 association with the CHA1 promoter was measured by ChIP using wild type (BY4742) and gal11/med15Δ med3Δ (LS10) yeast grown in CSM without (uninduced) or with (induced) 1 mg/ml of serine. B, histone H3 association with the indicated promoters was measured in wild type (RMY521) and srb4/med17 ts (RMY522) yeast grown in YPD after 1 h growth at 37 °C. C, histone H3 association with promoters whose transcription is dependent or independent of the Mediator tail module, as indicated, was measured in wild type (BY4742) and gal11/med15Δ med3Δ (LS10) yeast grown in YPD. Relative association in all cases was determined by quantitative PCR analysis of input and immunoprecipitated (IP) samples, and normalized to a non-transcribed region of ChrV (25). Error bars represent S.D. (n = 3).