FIGURE 5.
Dependence on Mediator for transcription, chromatin opening, and histone eviction of the PHO5v33 promoter. A, β-galactosidase activity (Miller units) was measured for wild type (BY4742) and gal11/med15Δ med3Δ (LS10) yeast harboring the plasmid pPpho5v33-lacZ and grown in raffinose-containing medium and induced by addition of galactose for the indicated times. Error bars represent S.D. (n = 3). B, ClaI accessibility of the PHO5v33 promoter was determined in nuclei prepared from strains and using growth conditions as in A. Biological replicates for the 30-min induction time point of wild type and mutant gave 88 and 94% ClaI accessibility, respectively. C, histone H3 association at the PHO5v33 promoter was measured by ChIP using strains and growth conditions as in A. Error bars represent the variation between two biological replicates.