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. 2014 Apr 11;289(21):14981–14995. doi: 10.1074/jbc.M113.529354

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Dependence of histone H3 association on the Swi/Snf complex. A, association of histone H3 with the CHA1 and SPO20 promoters was measured by ChIP in wild type (BY4741) or snf5Δ (yeast deletion collection) yeast grown in CSM under non-inducing conditions (no serine) or after induction for 15 or 30 min by addition of 1 mg/ml of serine. B, histone H3 association at the indicated promoters was measured using the samples from A obtained from yeast grown under non-inducing conditions for 30 min. Relative association in all cases was determined by quantitative PCR analysis of input and immunoprecipitated (IP) samples, and normalized to a non-transcribed region of ChrV (25). Error bars represent S.D. (n = 3).