FIGURE 4.

Expression of mortalinΔ51 in bacteria and in K562 cells. A, full-length mortalin or mortalinΔ51 in pETDuet in E. coli were induced by isopropyl β-d-thiogalactopyranoside, and the bacteria were lysed with sample buffer and analyzed by SDS-PAGE and Western blotting with anti-mortalin antibody. K562 cell lysate was analyzed together with them. B, K562 cells transfected for 48 h with pEGFP-N1 expressing mortalin-EGFP, mortalinΔ51-EGFP, or EGFP alone were lysed with sample buffer and analyzed by SDS-PAGE and Western blotting with anti-EGFP antibody. C, transfected K562 cells were analyzed under a confocal microscope, and a representative cell is shown. D, transfected K562 cells with mortalin-EGFP were treated with MitoTracker (25 nm) for 10 min at 37 °C to label their mitochondria. A confocal microscope image of a representative cell shows co-localization of mortalin with mitochondria.