FIGURE 8.

NaCl inhibits binding of mortalin to C9. A, human C9 (or BSA as control) (0.5 μg) was attached overnight to microtiter plate wells and then incubated with His-tagged mortalin in the presence of NaCl (75–375 mm) for 1 h in 37 °C. The wells were then washed, treated with anti-His antibody and peroxidase-labeled secondary antibody, developed with OPD, and analyzed in an ELISA reader. B, C9 or BSA (0.5 μg) attached to microtiter plate wells was incubated in the presence of NaCl (75–375 mm) without mortalin for 1 h in 37 °C. The wells were then washed, treated with anti-C9 antibody and peroxidase-labeled secondary antibody, developed with OPD, and analyzed in an ELISA reader. *, p < 0.05; **, p < 0.005 relative to control (C9 and mortalin without added NaCl). Error bars, S.D.