FIGURE 9.
Nucleotides inhibit binding of mortalin to complement C9. Human C9 attached to microtiter plate wells was incubated with His-tagged mortalin in the presence of ATP, ADP, or AMP-PNP (0.1–103 μm) for 1 h in 37 °C. The wells were then washed, treated with anti-His antibody and peroxidase-labeled secondary antibody, developed with OPD, and analyzed in an ELISA reader. Results are presented as percentage control of binding at 0.1 μm nucleotide. *, p < 0.05; **, p < 0.005 relative to 0.1 μm same nucleotide.