FIGURE 7.

Rad59 is essential for Rad52-mediated suppression of dna2-K1080E. A, the Rad52-dependent suppression of dna2-K1080E was examined in rad59-null cells. Overexpression of Rad52 in rad59-null cells is indicated as rad59Δ+Rad52. Transformants were grown and spotted as described above in legend for Fig. 1C. FOA, 5-fluorooritic acid. B, the pRS325-ADH1 plasmids expressing Dna2, Rad52, and Rad52-L89F were introduced into YJA1BJK65, and the resulting transformants were grown and spotted as described in the legend for Fig. 1A. C, expression levels of Rad52 and Rad52-L89F proteins were examined as described in legend for Fig. 1B. Histone H3 was used as loading control. α-H3, antibodies against histone H3.