FIGURE 8.

Rsc2 and Elg1 are essential for the Rad52-dependent suppression of dna2-K1080E. A, the Rad52-dependent suppression of dna2-K1080E was examined in rsc2- and rsc7-null cells. Overexpression of Rad52 in rsc2- or rsc7-null cells is indicated as rsc2Δ+Rad52 and rsc7Δ+Rad52, respectively. FOA, 5-fluorooritic acid. B, expression levels of Rad52 proteins were examined in the absence of Rsc2 or Rsc7 as described in the legend for Fig. 1B. C, overexpression of Elg1 suppresses the lethality of dna2-K1080E. The pRS325-ADH1 plasmids expressing Dna2 and Elg1 were introduced into YJA1BJK65 (dna2::HIS3, pRS314-dna2-K1080E, and pRS316-DNA2), and the resulting transformants were grown and spotted as described in the legend for Fig. 1A. D, the pRS325-ADH1 plasmids expressing Dna2 and Elg1 were introduced into YJA2 (dna2Δ405N). Cells were grown until saturated and spotted on an SD-L plate followed by incubation at 25 and 37 °C for 3 days. E, the Rad52-dependent suppression of dna2-K1080E was examined in elg1-null cells as described in the legend for Fig. 1C. Overexpression of in elg1-null cells is indicated as elg1Δ+Rad52. F, the Elg1-dependent suppression of dna2-K1080E was examined in rad52-null cells. Overexpression of Elg1 in rad52-null cells is indicated as rad52Δ+Elg1.