FIGURE 4.

Thermostability assay for tAlv-a1-pHCl and nAChR from T. californica. The Torpedo receptor was affinity-purified in complex with bungarotoxin in the detergent Cymal6. The tAlv-a1-pHCl receptor was solubilized with DDM and purified as described above. Torpedo nAChR was incubated for 10 min at 50 °C (lane 1) and at room temperature (lane 2). Alv-a1-pHCl was incubated for 10 min at room temperature and at 40, 50, 55, 65, and 70 °C (lanes 4–9) and for 20 min at 50 °C (lane 10). The samples were applied to a blue native PAGE with a 18–4% acrylamide gradient. The marker proteins (lane 3) were at 670, 440, 230, and 140 kDa.