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. 2014 Apr 9;289(21):15130–15140. doi: 10.1074/jbc.M113.525576

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Channel activity of Alv-a1-pHCl and tAlv-a1-pHCl observed in Xenopus oocytes. A, tAlv-a1-pHCl was expressed in Xenopus oocytes, and activation was tested with different neurotransmitters (the concentrations tested are indicated). Robust inward currents were only evoked by lowering the pH. B, the same for Alv-a1-pHCl. Control experiments with non-expressing oocytes were conducted at pH 4.7 (47 oocytes) and pH 3.0 (66 oocytes) and yielded average currents of −0.12 and −0.40 μA with a standard error of 0.09 and 0.20, respectively.