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. 2014 Jan 4;9:1. doi: 10.1186/1750-1326-9-1

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Copyright © 2014 Buggia-Prévot et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Axonal and presynaptic localization of BACE1-YFP in hippocampal mossy fibers of transgenic mice. (A) Schematic structure of tetO BACE1-YFP transgene. (B) BACE1 expression in mouse forebrain lysates from bigenic BACE1-YFP (Tg BACE1-YFP), CamKIIα-tTA, non-transgenic (WT), and BACE1−/− mice was analyzed by immunoblotting with mAb 3D5. (C) Localization of endogenous BACE1 and BACE1-YFP in presynaptic terminals of the mouse brain hippocampal mossy fibers (MF). Top, non-transgenic mouse brain stained with anti-BACE1 mAb 3D5. 3 month-old non-transgenic (top panel) or Tg BACE1-YFP mouse brains (bottom) were stained with antibodies against synaptophysin, and MAP2 and were analyzed by confocal microscopy. Neuronal cell bodies in dentate gyrus (DG) were only weakly positive for BACE1-YFP.