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. Author manuscript; available in PMC: 2014 May 23.
Published in final edited form as: Cell Rep. 2014 Apr 24;7(3):681–688. doi: 10.1016/j.celrep.2014.03.048

Figure 2. Botch GGCT like activity is required for regulation of embryonic neurogenesis in vivo.

Figure 2

(A) A schematic diagram of pCAG constructs for overexpression (gain of function) for in utero injection and electroporation.

(B–E) Distribution of GFP+ cells 2 days after in utero injection and electroporation.

(B) Representative confocal images of cortex immunostained for GFP with and without the DAPI channel with Botch expression. Abbreviations: CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone; SVZ, subvetricular zone.

(C) Quantification of distribution of GFP+ cells in (B). Values represent the mean ± SEM (n ≥ 3; ** p < 0.01; *** p < 0.001; n.s. p > 0.05, one-way ANOVA, post-test: Tukey’s multiple comparison tests).

(D) Representative confocal images of cortex immunostained for GFP with and without the DAPI channel following knockdown of Botch and rescue either with BotchR or BotchR-E115A.

(E) Quantification of distribution of GFP+ cells in (D). Values represent the mean ± SEM (n ≥ 3; *** p < 0.001; n.s. p > 0.05; one-way ANOVA, post-test: Tukey’s multiple comparison test).