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. Author manuscript; available in PMC: 2014 May 23.
Published in final edited form as: Nature. 2013 Mar 24;496(7444):238–242. doi: 10.1038/nature11986

Fig. 2. HIF-1α is responsible for LPS-induced IL-1β expression.

Fig. 2

(A) LPS-induced HIF-1α and IL-1β protein expression ± 2DG (1, 5, 10mM). (B) IL-1β in BMDMs incubated in normoxia or hypoxia for 24h then LPS for 24h. (C) IL-1β mRNA in LPS-stimulated BMDMs pretreated ± αKG derivative27 (1mM). (D) LPS-induced HIF-1β and IL-1β in BMDMs pretreated ± αKG (0.01, 1, 100, 1000μM). (E) LPS-induced IL-1β protein in WT and HIF-1α-deficient BMDMs. (F) ChIP-PCR using HIF-1α antibody and primers specific for −300 position of il1b in LPS-treated BMDMs ±2DG (1mM). (G) Reporter activity in RAW-264 cells transfected with il1b- or −357 il1b. Representative of 3 experiments. Error bars, s.d. (I) ChIP-PCR as above in BMDMs treated with LPS ±αKG (1mM). Error bars, s.e.m, ** p < 0.01.