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. Author manuscript; available in PMC: 2014 May 23.
Published in final edited form as: J Biol Chem. 2003 Mar 26;278(22):20405–20412. doi: 10.1074/jbc.M211795200

Fig. 1. In vivo interaction of Myc with various subunits of the human STAGA complex requires its N-terminal amino acids 1-110.

Fig. 1

A, lysates of HEK293 cells (lanes 1 and 2) were immunoprecipitated with non-immune rabbit serum (NRS, lane 1) or with rabbit polyclonal antibodies to Max (C-17) (lane 2), and immunoprecipitated proteins were detected by Western blot with specific antibodies for the indicated proteins. Lane 3 contains STAGA subunits immunoprecipitated with a FLAG antibody from cells expressing FLAG/HA-GCN5L (fh:GCN5L). Different strips of the same blot are shown. B, lysates of HEK293 cells transfected with pCbS (CMV vector; lanes 1 and 2), pCbS/FLAG-c-Myc (lanes 3 and 4), and pCbS/FLAG-c-Myc(Δ1–110) (lanes 5 and 6) were immunoprecipitated (IP) with an anti-FLAG antibody and bound proteins were analyzed by Western blot (WB). The upper panels show the immunoprecipitated proteins in different strips of the same blot probed with the indicated antibodies. Similarly, the bottom panels show the corresponding proteins in the input lysates used for immunoprecipitation. The strips probed with GCN5 antibody were reprobed (after stripping) with TAF5 antibodies.