Table 2.
Example Yeast Proteins Identified by Phosphoprotein Enrichment Followed by by Top-Down and Bottom-Up Proteomics
| UniProt # | Protein | Measured Mr (Da) |
Theoretica l Mr (Da) |
MMA (ppm) |
Modifications |
|---|---|---|---|---|---|
| YBR085C-A | Uncharacterized protein YBR085C-A | 9023.2383 | 9023.2117 | 2.95 | 5 phosphorylation, loss of N-term 7 residue |
| YBR085C-A | Uncharacterized protein YBR085C-A | 9714.5522 | 9714.5658 | −1.40 | 5 phosphorylation, 1 acetylation, loss of N-term Met |
| YCR031C | 40S ribosomal protein S14-A (RP59A) | 14700.7195 | 14700.7173 | 0.15 | 1 phosphorylation, 2 acetylation |
| YDR382W | 60S acidic ribosomal protein P2-beta (P2B) | 11209.3462 | 11209.3256 | 1.84 | 2 phosphorylation |
| YDR424C | Dynein light chain 1, cytoplasmic | 10347.2519 | 10347.2927 | −3.94 | 3 phosphorylation, loss of N-term 3 residues |
| YDR424C | Dynein light chain 1, cytoplasmic | 10629.2439 | 10629.318 | −6.97 | 4 phosphorylation, loss of N-term Met |
| YFL014W | 12 kDa heat shock protein | 11682.6032 | 11682.6114 | −0.70 | 1 phosphorylation,1 acetylation, loss of N-term Met |
| YFL014W | 12 kDa heat shock protein | 11602.6226 | 11602.6451 | −1.94 | 1 acetylation, loss of N-term Met |
| YGR035C | Uncharacterized protein YGR035C | 13137.3846 | 13137.3944 | −0.75 | 4 phosphorylation, 1 acetylation, loss of N-term Met |
| YHL015W | 40S ribosomal protein S20 | 13986.5802 | 13986.5342 | 3.29 | 1 phosphorylation |
| YHR132W-A | Protein IGO2 | 13510.6029 | 13510.6100 | −0.53 | 5 phosphorylation, 1 methylation, loss of N-term 12 residue |
| YIL138C | Tropomyosin-2 | 19101.1719 | 19101.1965 | −1.29 | 3 phosphorylation, 2 methylation, loss of N-term 2 residue |
| YLL050C | Cofilin (Actin-depolymerizing factor 1) | 16276.7906 | 16276.8377 | −2.89 | 4 phosphorylation, 1 acetylation, 1 methylation |
| YLR390W-A | Covalently-linked cell wall protein 14 (Inner cell wall protein) | 23484.6962 | 23484.8575 | −6.87 | 2 phosphorylation, 1 acetylation, 1 methylation |