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. 2014 Jun;55(6):1019–1032. doi: 10.1194/jlr.M039925

Fig. 2.

Fig. 2.

Rohitukine inhibits adipogenesis. A: 3T3-L1 preadipocytes and C3H10T1/2 were cultured in the DMEM and MDI medium containing 0, 5, 10, 15, and 20 μM rohitukine for 8 days. Culture dish images were acquired after lipid droplet staining with ORO; microscopic images were taken with Nikon Eclipse TI. Absorbance of extracted ORO accumulated in lipid droplets of 3T3-L1-derived (B) and C3H10T1/2-derived (C) adipocytes was measured spectrophotometrically at 492 n. D: 3T3-L1 preadipocytes and C3H10T1/2 were cultured in the MDI medium with 20 μM rohitukine for 0–2, 0–4, 0–6, 2–4, 2–6, and 4–6 days. The lipid droplets were stained with ORO, and microscopic images were taken with Nikon Eclipse TI. Absorbance of extracted ORO accumulated in lipid droplets of 3T3-L1-derived (E) and C3H10T1/2-derived (F) adipocytes was measured spectrophotometrically at 492 nm. All values are presented as the mean ± SD of three experiments performed in triplicate. Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.