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. 2014 Jun;55(6):1019–1032. doi: 10.1194/jlr.M039925

Fig. 3.

Fig. 3.

Molecular markers of adipogenic inhibition. A: Relative mRNA abundance of LPL, aP2, SREBP-1c, FAS, PPARγ, and C/EBPα on day 6 of adipocyte differentiation was measured by real-time PCR in the absence and presence of 20 μM rohitukine in MDI. B: Rohitukine addition to MDI significantly downregulates the late phase protein level expression of GLUT-4, C/EBPα, PPARγ, aP2, and FAS in 3T3-L1 adipocytes (i.e., on day 2, day 4, and day 6 of differentiation). C: Rohitukine inhibits early phosphorylation states of p-AKT (Ser473), mTOR (Ser2448), and 4EBP (Thr37/46) observed by Western blotting. D: Rohitukine increases the relative mRNA abundance of Wnt3a and GATA2 on day 2 measured by real-time PCR. The results were verified by three independent experiments, mean ± SD. Significance of difference between the MDI-induced control and rohitukine-treated groups: * P < 0.05, ** P < 0.01, *** P < 0.001.