TABLE 1.

Rate and equilibrium constants, as well as thermodynamic parameters (in kJ mol⁻¹), for the interaction of DHE with the lipoproteins at 35°C. The parameters for DHE interaction with lipid vesicles were taken from literature (11) and are also shown.

	HDL3	HDL2	LDL	VLDL	POPC:Chol 1:1	SM:Chol 6:4
kdiff (1010 M−1 s−1)	2.1	2.9	5.6	14	25	25
k−diff (106 s−1)	143	66	16	2.5	0.77	0.77
k+ (106 M−1 s−1)	3.4 ± 1.2	3.0 ± 1.5	4.1 ± 1.2	21 ± 8	64 ± 6	13 ± 1
kin (102 s−1)	234 ± 84	67 ± 34	12 ± 4	3.9 ± 1.5	2.0 ± 0.2	0.40 ± 0.03
k− (10−3 s−1)	30 ± 8	25 ± 9	2.5 ± 1.1	1.7 ± 0.9	0.6 ± 0.2	0.07 ± 0.02
$K_{\text{P}}^{{\text{L}}_x/\text{W}}$ (105)a	11 ± 5	4.2 ± 0.9	11 ± 6	12 ± 10	25 ± 8	47 ± 15
ΔH°(partition)	19 ± 9	4 ± 5	−42 ± 13	24 ± 30	−13 ± 10	53 ± 7
ΤΔS°(partition)	54 ± 10	37 ± 5	−6 ± 13	59 ± 30	23 ± 10b	90 ± 7b
ΔH‡°(insertion)c	130 ± 10	116 ± 14	90 ± 7	95 ± 15	83 ± 7	110 ± 14
ΤΔS‡°(insertion)c	93 ± 10	79 ± 15	54 ± 7	63 ± 15	53 ± 7	77 ± 15
ΔH‡°(desorption)c	111 ± 7	111 ± 10	132 ± 9	72 ± 21	95 ± 12	57 ± 16
ΤΔS‡°(desorption)c	27 ± 7	26 ± 11	41 ± 9	−20 ± 20	1 ± 12	−43 ± 16

^aThe partition coefficient between the aqueous phase and the lipid monolayer at the lipoproteins surface was calculated from the respective equilibrium binding constant as previously described (14).

^bThis entropy variation is different from the values given in the original reference because it is calculated from the temperature dependence of K_P (instead of $K_{{\text{L}}_{\text{V}}}$) to allow comparison between the parameters obtained for binding agents with very distinct sizes.^{c ‡} refers to the thermodynamic parameters of the transition state in the kinetic steps.