TABLE 1.

Lipase activity of S4-3, S4-3M, and S4-3N against the triglyceride substrates

Triacylglycerols	S4-3 (U/mg)	S4-3M (U/mg)	S4-3N (U/mg)
Triacetin (C2)	14.2 ± 0.5	47.4 ± 5.6	532.9 ± 35.9
Tributyrin (C3)	66.3 ± 1.2	54.4 ± 3.7	476.2 ± 32.8
Tricaproin (C6)	2,550.6 ± 58.2	7,911.6 ± 230.9	422.9 ± 45.3
Tricaprylin (C8)	8,394.6 ± 250.0	14,196.1 ± 259.8	258.4 ± 24.8
Trilaurin (C12)	9,504.2 ± 280.1	14,374.4 ± 330.1	120.1 ± 15.7
Tripalmitin (C16)	3,281.6 ± 64.6	5,858.9 ± 78.2	8.2 ± 2.1
Tristearin (C18)	1,121.5 ± 49.4	678.5 ± 34.5	5.9 ± 1.3

The reaction conditions were pH 8.0 and 40°C for S4-3 and S4-3M, and pH 8.0 and 45°C for S4-3N, respectively.