TABLE 2.

Interfacial kinetic parameters of S4-3, S4-3M, and S4-3N against p-nitrophenyl esters

Enzymes	pNPL (C12)			p-Nitrophenyl Acetate (C2)			p-Nitrophenyl Propionate (C3)
	k*cat	K*m	k*cat /K*m	k*cat	K*m	k*cat /K*m	k*cat	K*m	k*cat /K*m
	(s−1)	(μM)	(M−1 s−1)	(s−1)	(μM)	(M−1 s−1)	(s−1)	(μM)	(M−1 s−1)
S4-3	27.2 ± 2.8	398 ± 9	6.8 × 104	5.9 ± 0.9	1,620 ± 12	3.6 × 103	7.8 ± 0.8	1,550 ± 15	5.0 × 103
S4-3M	168.4 ± 2.2	455 ± 12	3.7 × 105	6.2 ± 0.7	1,580 ± 15	3.9 × 103	6.9 ± 0.6	1,650 ± 18	4.2 × 103
S4-3N	4.5 ± 0.7	1,903 ± 15	2.4 × 103	21.3 ± 0.6	320 ± 10	6.7 × 104	23.1 ± 0.7	350 ± 12	6.6 × 104

The reaction conditions were pH 8.0 and 40°C for S4-3 and S4-3M, and pH 8.0 and 45°C for S4-3N, respectively.