Fig. 7.

Native FASN activity is decreased in Spot14-null MEC cytosolic lysates. A: [¹³C] fatty acid products of enzyme catalysis were quantified using GC-MS. FASN activity increased 1.6-fold in control lysates that contained Spot14 relative to Spot14-null lysates, and add back of Spot14 in 40 molar excess to null lysates increased catalysis approximately 20% under reaction conditions containing 20 μM [¹³C]₂-acetyl-CoA and [¹³C]₃-malonyl-CoA. B: Cytosolic lysates from control MECs had a significantly increased reaction velocity for overall enzyme kinetics (P = 0.005). Add back of recombinant Spot14 in 40× molar excess to null lysates trended higher but did not reach significance (P = 0.08).