TABLE 3.
The concentration of sEH in the S9 fraction of pooled (4–50 persons) human tissues (Xenotech LLC, Lenexa, KS)
| Tissue | [Protein] (mg ml−1) | [3H]t-DPPO Specific Activity (nmol·min−1·mg−1) | [sEH] (nM) | |
| Estimated from Activity | Estimated from Western Blot | |||
| Liver | 16.98 | 8.50 ± 1.80 | 420 ± 89 | 500 ± 150 |
| Kidney | 4.15 | 3.67 ± 0.29 | 44 ± 3 | 80 ± 15 |
| Lung (N.S.) | 4.92 | 0.21 ± 0.02 | 3.0 ± 0.3 | 22 ± 6 |
| Lung (S.) | 4.49 | 0.22 ± 0.02 | 2.8 ± 0.3 | 21 ± 4 |
| Intestine | 4.23 | 6.53 ± 0.71 | 80 ± 9 | 110 ± 10 |
N.S., nonsmoker; S., smoker. Concentrations estimated from the specific activity using [3H]t-DPPO as substrate ([S]final= 50 µM) and by Western blot using recombinant purified human sEH as a standard. Results are average ± SD (n = 3).