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. 2014 Jun;55(6):1150–1164. doi: 10.1194/jlr.M047357

Fig. 6.

Fig. 6.

Effect of EPA/DHA supplementation on leukotriene and prostanoid formation after calcium ionophore stimulation. The levels of free metabolites were determined after A23187-mediated stimulation of whole blood samples and thus reflect the metabolic capacity and substrate specificity of the corresponding enzymes expressed in blood cells. A, B: Generated levels of AA-derived LTB4 and EPA-derived LTB5 and the relative abundance of LTB5 and LTB4 in correlation with the EPA/AA precursor fatty acid ratio. C, D: Levels of AA-derived PGE2 and EPA-derived PGE3 and the relative abundance of PGE3 and PGE2 in correlation with the EPA/AA precursor fatty acid ratio. E, F: Levels of AA-derived TXB2 and EPA-derived TXB3 and the relative abundance of TXB3 and TXB2 in correlation with the EPA/AA precursor fatty acid ratio. Data are given as mean ± SEM, n = 19. A general linear model for repeated measurements was used for analysis (A, C, E) and significant changes are indicated as: *P < 0.05 versus basal level [week 0 (W0)] and #P < 0.05 versus maximum treatment [week 8 (W8)]. For relative efficiencies, a Pearson/Spearman Rho correlation was performed: LTB5/LTB4, y = 0.58x − 0.01, r = 0.864 with P < 0.001 (B); PGE3/PGE2, y = 0.16x − 0.002, r = 0.712 with P < 0.001 (D); TXB3/TXB2 no correlation (F).