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. Author manuscript; available in PMC: 2014 May 23.
Published in final edited form as: J Comp Neurol. 2011 Dec 1;519(17):3532–3548. doi: 10.1002/cne.22721

Figure 9.

Figure 9

Delayed differentiation of progenitors in the NFIX−/− cerebellum. Expression of the neural progenitor cell marker nestin (A–D) and the astroglial marker GLAST (E–H) in the cerebellum of wild-type and NFIX−/− mice at P5. In the wild-type at P5, expression of nestin was low, with expression observed on radial fibers of progenitors at the pial surface of the cerebellum (arrowheads in B). In the mutant, however, expression of nestin was markedly higher (arrowheads in D). Conversely, expression of GLAST by Bergmann glia was markedly higher in the wild type (arrows in F) compared with the NFIX knockout cerebellum (arrows in H). B,D,F,H are higher magnification views of the boxed regions (lobules IV–VI) in A,C,E,G, respectively. I,J: qPCR on wild-type and NFIX−/− P5 cerebellar tissue demonstrated significantly increased levels of nestin (I) and significantly reduced levels of Glast (J) mRNA in the mutant. *P < 0.05, t-test. Scale bar = 20 µm in H (applies to B,D,F,H); 100 µm for A,C,E,G.