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( A and B) Confocal images of acid-treated fibroblasts zero and seven days after culture. None of the acid-treated fibroblasts expressed the Oct4-GFP transgene. ( C) qPCR analysis demonstrated that acid-treatment did not induce Oct4, Sox2 and Nanog expression. No expression was determined after 40 cycles of qPCR for Oct4 and Sox2, hence marked as ‘undetermined’. Error bars represent standard error of the mean (p≤0.05).
