Fig. 1.

Chromosomal locations and gene order of CG9897, CG32833, and CG32834 and their orthologs in Drosophila species. In D. simulans, GD15206 is found in an unassembled part of chromosome (chr) 2R (indicated by the asterisk), whereas UN32833 and UN9897 represent unannotated copies whose sequences we determined by sequencing or BLAST. The Dsim\UN32833 sequence is only partially determined (the 142 codons at the start of the coding sequence). Color indicates gene expression pattern: pink is female-specific or female-biased; bright blue is male specific; light blue is expressed only at a low level in males; green is expressed approximately equally in both sexes. Gene order and conserved expression patterns were consistent with calls of orthology and with phylogenetic clustering (see fig. 4).