Fig. 3.

Tissue-specific expression patterns of Drosophila melanogaster CG32834, CG32833, and CG9897 and their orthologs in D. ananassae. Expression patterns are also shown for GA25104, the single D. pseudoobscura member to the gene family. L: ladder; B: body without RT; G: gonads; R: RT without gonads; g: genomic DNA; and N: negative control (water used as template). RpL32 expression was used as a control. Notes: (a) At 35 cycles, a light band appears in the male gonads and RTs without gonads of Dmel for CG9897. (b) GF11310 is a D. ananassae homolog to the gene family but is not definitively orthologous with any one gene. GA25104 is a D. pseudoobscura homolog to the gene family but is not definitively orthologous with any one gene.