Table 2.
Trial 1a |
Trial 2b |
|||||
---|---|---|---|---|---|---|
Gene that was knocked down | 1 day | 4 days | 10 days | 1 day | 4 days | 10 days |
CG32833 | n.s.c | n.s. | n.s. | No datac | No data | No data |
CG9897 | n.s. | = 6.0* | n.s. | n.s. | = 6.5** | = 27.2*** |
CG32834 | n.s. | n.s. | = 3.9* | = 3.7* | n.s. | = 4.7* |
CG9897 and CG32834 | n.s. | n.s. | = 4.9* | n.s. | n.s. | = 7.6** |
CG9897 and CG32833 | n.s. | n.s. | n.s. | No data | No data | No data |
CG32834 and CG32833 | n.s. | n.s. | n.s. | No data | No data | No data |
CG9897, CG32834, and CG32833 | n.s. | n.s. | n.s. | No data | No data | No data |
Note.—Data were analyzed using a generalized linear model with a logit link in JMP. Variables were eliminated using backward iteration until only variables with P values ≤ 0.15 remained.
aIn Trial 1, control females or females knocked down for each female gene (individually and in combination) were mated to control males or males knocked down for CG32833 for the initial mating (on Day 0) and then tested for remating with a Canton S male on the indicated day. Day 1: N = 17–37 females per treatment; Day 4: N = 15–37 females per treatment; Day 10: N = 73–88 females per treatment (three replicates combined).
bIn Trial 2, control females or females knocked down for each female gene (individually and in combination) were mated to wild-type males (Canton S) for the initial mating (on Day 0) and then tested for remating with a Canton S male on the indicated day. Day 1: N = 37–45 females per treatment (two replicates combined); Day 4: N = 35–53 females per treatment (two replicates combined); Day 10: N = 88–108 females per treatment (four replicates combined).
cn.s.: not significant; no data: not tested.
*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.