Table 1.
List of primers used in the present study
| Gene/name | Sequence 5’-3’ | Tm (°C) | Amplicon length |
|---|---|---|---|
| RPDEFAF |
GAATACTCCACTCAACCGCAAC |
62.7 |
|
| RPDEFAR |
TAGTTCCTTTACATCGGCCA |
58.4 |
295 bp |
| RPDEFBF |
CAGTACCTAGGATATTCCACTCAAC |
62.9 |
|
| RPDEFBR |
TAGTTCCTTTACAATGGCCG |
58.4 |
304 bp |
| RPDEFCF |
CAGTACAGTCCTAATACCTAGCC |
62.8 |
|
| RPDEFCR |
CAGTTCCTACGCAACGGCCT |
64.5 |
300 bp |
| RPLYS1F |
TTCTTACTGGCTATTTTCGCC |
58.7 |
|
| RPLYS1R |
CGACCTCTGCAATGGTACTG |
62.4 |
377 bp |
| RPLYS2F |
CTAGTTTTAACACTATTGCTGCTG |
59.4 |
|
| RPLYS2R |
GCCCTTACATTTCTTGATCC |
58.4 |
378 bp |
| RPPROLF |
CTATAACGAGTGAACTATAAGACAA |
50.0 |
|
| RPPROLR |
GTGTTTAATGGCGGTAACAAATTAC |
53.2 |
406 bp |
| RPACTF |
CACGAGGCTGTATACAATTCCA |
60.8 |
|
| RPACTR | GTAGCTGTTTAGAAGCATTTGCG | 61.0 | 314 bp |