Table 2. Protection of SNAP-25 by Steroidal SMNPIsa.
| % of intact SNAP-25 |
|||
|---|---|---|---|
| compd | A | B | C |
| 36 | 16 | 28 | 36 |
| 60 | 89 | 96 | 93 |
| 52 | 77 | 88 | 81 |
| blank | 31b | 40 | 43 |
a
A: 488.5 μL of medium + 1.5 μL (10 mM) of compound + 10 μL of BONT-A (50 nM). B: 483.5 μL of medium + 1.5 μL (10 mM) of compound + 5 μL of Baf (100 nM) + 10 μL of BONT-A (50 nM). C: 463.5 μL of medium + 1.5 μL (10 mM) of compound + 25 μL of Baf (100 nM) + 10 μL of BONT-A (50 nM). Blank: 490 μL of medium + 10 μL if BONT-A (50 nM).
b
Average value from four measurements.