Figure 5. Transport of Acyclovir by hOAT2-HEK293 Cells.

The uptake kinetics of [³H]-acyclovir were evaluated in OAT2-transfected HEK293 cell lines using a 2 minute incubation period. Uptake of [³H]-acyclovir was substantially greater than that of [³H]-PAH in the OAT2 expressing cells, but not the control (empty vector) cells (A). The kinetics of OAT2-HEK293 cell mediated transport of [³H]-acyclovir transport was high-capacity and moderate affinity (B). Acyclovir uptake was greatest in cells expressing OAT2 in comparison to cells expressing OAT1, OAT3 or OAT4 under the same experimental conditions (C). Values represent mean ± SEM of at least three replicate determinations in a single experiment.