Figure 2. Relative amounts of transcripts for Toll-like receptor (TLR), Toll-like Receptor associated factor 6-like protein (TRAF 6), Macrophage-Expressed Gene protein (MPEG), caspase-like 3/7, BCl-2 homolog protein-1 (BHP-1) and BCl-2 homolog protein-2 (BHP-2) in N-3-oxododecanoyl-L-homoserine lactone (3-oxo-C12-HSL)-stimulated Suberites domuncula compared to control S. domuncula.
The mRNA levels in 8 h-stimulated S. domuncula were evaluated by quantitative reverse transcription-PCR and compared to those in control sponges. Reactions were performed in triplicate with three different specimens. Relative mRNA level values resulted from calculating values: values above and below 1 show a higher and a lower mRNA level in the presence of 10−6 M 3-oxo-C12-HSL, respectively. Significant expression changes (p<0.05) in the presence of N-3-oxododecanoyl-L-homoserine are pointed out by an asterisk.