Table 1. MS identification of the proteins from total primmorph extract and from the membrane fractions, which displayed differential abundances in N-3-oxodecanoyl-_L-homoserine lactone (3-oxo-C₁₂-HSL)-stimulated Suberites domuncula primmorphs compared to control primmorphs (p<0.05).

Protein spots		Protein analysis					Protein identification
protein number regulationa		sequencebankb	GenBank accession number	Mascot score	recognized peptide numbers	sequence coverage (%)	homology (species)	E-Value
24 h-stimulated primmorphs (total soluble protein fraction)
17	+	S. domuncula	gi|300467112	59	1	11
39	+
70	+
99	+	S. domuncula	gi|300479356	93	2	10	α-actinin 1 (Homo sapiens)	5. 10−68
102	+
104	+	S. domuncula	gi|300478742	167	4	13	α subunit lysosomal ATPase (Homo sapiens)	5. 10−75
114	+
121	+	S. domuncula	gi|300479590	172	3	16
143	+
154	+	A. queenslandica	Aqu1.228276	83	3	1	β subunit lysosomal ATPase (Homo sapiens)	8. 10−67
157	+	A. queenslandica	Aqu1.228276	84	4	1	β subunit lysosomal ATPase (Homo sapiens)	8. 10−67
169	+	S. domuncula	gi|300467815	125	3	17	tubulin α-1 chain (Parentrotus lividus)	1. 10−124
24 h-stimulated primmorphs (membrane soluble protein fraction)
394	+	S. domuncula	gi|300469085	143	4	26	actin (Aedes aegypti)	2. 10−132
503	+	S. domuncula	gi|300465889	74	2	12	cognin (Gallus gallus)	5. 10−58
1201	+	S. domuncula	gi|300465842	78	3	11	cofilin/Actin depolymerizing factor (Grosmannia clavigera)	1. 10−15
1402	+
3608	−
4204	+
4502	−	S. domuncula	gi|300467049	128	4	23	α-tubulin (Suberites ficus)	6. 10−137
4504	+	S. domuncula	gi|300468062	67	1	4	cofilin/Actin depolymerizing factor (Grosmannia clavigera)	1. 10−15
5202	−

^aThe amount of proteins in fractions is higher (+) or lower (−) in presence of 3-oxo-C₁₂-HSL.

^bProtein MS spectra were compared to theoretical spectra of proteins generated from S. domuncula and Amphimedon queenslandica EST data bases.