Table 1. MS identification of the proteins from total primmorph extract and from the membrane fractions, which displayed differential abundances in N-3-oxodecanoyl-L-homoserine lactone (3-oxo-C12-HSL)-stimulated Suberites domuncula primmorphs compared to control primmorphs (p<0.05).
Protein spots | Protein analysis | Protein identification | ||||||
protein number regulationa | sequencebankb | GenBank accession number | Mascot score | recognized peptide numbers | sequence coverage (%) | homology (species) | E-Value | |
24 h-stimulated primmorphs (total soluble protein fraction) | ||||||||
17 | + | S. domuncula | gi|300467112 | 59 | 1 | 11 | ||
39 | + | |||||||
70 | + | |||||||
99 | + | S. domuncula | gi|300479356 | 93 | 2 | 10 | α-actinin 1 (Homo sapiens) | 5. 10−68 |
102 | + | |||||||
104 | + | S. domuncula | gi|300478742 | 167 | 4 | 13 | α subunit lysosomal ATPase (Homo sapiens) | 5. 10−75 |
114 | + | |||||||
121 | + | S. domuncula | gi|300479590 | 172 | 3 | 16 | ||
143 | + | |||||||
154 | + | A. queenslandica | Aqu1.228276 | 83 | 3 | 1 | β subunit lysosomal ATPase (Homo sapiens) | 8. 10−67 |
157 | + | A. queenslandica | Aqu1.228276 | 84 | 4 | 1 | β subunit lysosomal ATPase (Homo sapiens) | 8. 10−67 |
169 | + | S. domuncula | gi|300467815 | 125 | 3 | 17 | tubulin α-1 chain (Parentrotus lividus) | 1. 10−124 |
24 h-stimulated primmorphs (membrane soluble protein fraction) | ||||||||
394 | + | S. domuncula | gi|300469085 | 143 | 4 | 26 | actin (Aedes aegypti) | 2. 10−132 |
503 | + | S. domuncula | gi|300465889 | 74 | 2 | 12 | cognin (Gallus gallus) | 5. 10−58 |
1201 | + | S. domuncula | gi|300465842 | 78 | 3 | 11 | cofilin/Actin depolymerizing factor (Grosmannia clavigera) | 1. 10−15 |
1402 | + | |||||||
3608 | − | |||||||
4204 | + | |||||||
4502 | − | S. domuncula | gi|300467049 | 128 | 4 | 23 | α-tubulin (Suberites ficus) | 6. 10−137 |
4504 | + | S. domuncula | gi|300468062 | 67 | 1 | 4 | cofilin/Actin depolymerizing factor (Grosmannia clavigera) | 1. 10−15 |
5202 | − |
The amount of proteins in fractions is higher (+) or lower (−) in presence of 3-oxo-C12-HSL.
Protein MS spectra were compared to theoretical spectra of proteins generated from S. domuncula and Amphimedon queenslandica EST data bases.