Figure 4. Effect of Pannexin 1 inhibitors on [ATPe] kinetics of a highly enriched population of trophozoite infected erythrocytes.

A. The time course of [ATPe] (pmol/10⁶ cells) was assessed for trophozoite-infected erythrocytes at 94% parasitemia (denoted as t94-RBCs) in the absence and presence of 100 µM carbenoxolone (CBX), 100 nM mefloquine (MFQ), or 100 µM of 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), inhibitors of Pannexin 1. Exposure to 3V is indicated by the arrow. In some experiments, prior to 3V exposure cells were pre-incubated 10 min with either CBX, MFQ or NPPB. For a comparison, similar experiments with noninfected RBCs (h-RBCs) are shown. t94-RBCs (N = 14, n = 19), t94-RBCs +CBX (N = 6, n = 7), t94-RBCs+MFQ (N = 4, n = 4), t94-RBCs+NPPB (N = 4, n = 4), h-RBCs (N = 15; n = 19), h-RBCs+CBX (N = 6, n = 9), h-RBCs+MFQ (N = 4, n = 4), h-RBCs+NPPB (N = 3, n = 3). N = independent preparations, n = replicates. B. 3V-dependent increase of [ATPe] calculated from A. Values are expressed as ΔATP₁, i.e., the difference between [ATPe] at 1 min post-stimulus and basal [ATPe]. Results are means ± SEM. (*p<0.05, ***p<0.001).