Figure 2. Lung mRNA expression levels of A) eNOS, K_Ca2.1, K_Ca2.2, K_Ca2.3, K_Ca3.1, and K_Ca1.1; B) α-Smooth muscle actin (α-SMA), collagen-1, and TGFβ.

Data are given as means ±SEM, n = 7–8. Data were analyzed by two-way ANOVA and differences were considered significant when *P<0.05 from wild type, ^# P<0.05 from normoxia. Statistical interaction (£) was observed in K_Ca2.3 expression (A). C–E) Genotyping: C:Gel electrophoresis shows that polymerase chain reacton (PCR) detected the K_Ca2.3-wild type allele (wild type (+)) and the tTA allele (T) in K_Ca2.3^T/+, and K_Ca2.3^T/T. D and E: PCR detected the targeted allele in K_Ca3.1^−/+ and in K_Ca3.1^−/− as well as the wild type allele in K_Ca3.1^+/− and K_Ca3.1^+/+. A DNA ladder was used to determine products sizes.