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. 2014 May 23;9(5):e97383. doi: 10.1371/journal.pone.0097383

Figure 4. Sulpiride decreases anterior pituitary cell proliferation in vivo.

Figure 4

Ovariectomized rats (n = 5–7 rats/group) were injected with sulpiride (5 mg/kg, 6 h) or saline, and with BrdU (50 mg/kg, 6 h). A: Sulpiride treatment induced hiperprolactinemia in OVX rats. Each column represents serum PRL levels ± SEM in CTRL or sulpiride-treated animals. *p<0.05 vs. CTRL animals injected with vehicle, Student t test. B: Proliferation rate determined by the detection of BrdU incorporation and FACS. Each column represents the mean ± SEM of the percentage of BrdU-positive cells. *p<0.05 vs. CTRL animals injected with vehicle, Student t test. C: Apoptosis was determined by FACS, using PI. Each column represents the mean ± SEM of the percentage of sub-G0-G1 cells. **p<0.01 vs. CTRL animals injected with vehicle, Student's t test. D: Representative histograms showing hypodiploidy in CTRL and sulpiride-treated animals.