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. 2014 May 23;9(5):e98034. doi: 10.1371/journal.pone.0098034

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© 2014 Laurent et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Effect of an anti-ICAM-1 antibody on cancer-EC interactions. Rupture force distributions are Gaussian with one or two peaks revealing the presence of receptor/ligand bonds or non specific interactions. Probability histograms of rupture force (V = 5 µm/s) for (A) T24-HUVEC, (B) J82-HUVEC, (C) RT112-HUVEC. Black histograms represent interaction cancer-cell and EC without antibody whereas red ones show the force distribution after using the antibody. Panels D (T24-ICAM-1) and E (T24-BSA) show the rupture force probabilities for T24 cells in contact with coated substrates. The number N of events is indicated on the histograms.