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. 2014 May 23;9(5):e98185. doi: 10.1371/journal.pone.0098185

Figure 1. Effect of bupivacaine on intracellular ATP levels and AMPK activation.

Figure 1

Neuro2a cells were treated with 2(A) Intracellular levels of ATP were measured with a luminescence assay (n = 4 per group). (B) Expression of phospho-AMPKα (P-AMPKα) and AMPK following cell exposure to bupivacaine was measured by Western blotting (n = 3 per group). ***P<0.001 vs. control (not treated with bupivacaine). All post hoc pairwise comparisons were performed by Tukey's test with repeated measures one-way ANOVA.