Figure 2. Suppression of the plant resistance by PsCRN70.

A. Lesions on the N. benthamiana leaves expressing PsCRN70 or GFP. A decolorized infected leaf was photographed 4 dpi. The experiments were repeated three times and shown with a representative image. B. Average lesion diameters on N. benthamiana leaves expressing the indicated genes inoculated with P. parasitica. Averages were calculated from four lesions per construct. Error bars represent standard errors. Different letters at the top of the columns indicate significant differences (P<0.01, Duncan's multiple range test). C. Phenotypes of the PsCRN70- and GFP-transgenic lines inoculated with P. parasitica. Detached leaves from five-weak old seedlings (T1 generation) of transgenic plants (#1, #3, #4, #12) were inoculated with the P. parasitica zoospores. Photographs were taken 36 hpi under a UV lamp. D. Lesion diameters on inoculated leaves measured 36 hpi. The experiments were repeated four times in all transgenic lines with similar results. Bars represent the standard deviation (SD). Different letters at the top of the columns indicate significant differences (P<0.01, Duncan's multiple range test). E. Phenotypes of the PsCRN70-transgenic plants inoculated with P. parasitica zoospores. The transgenic lines were inoculated with zoospores using the root dip method. Mock-inoculated seedlings were inoculated with sterile distilled water. Photographs were taken 4 days post inoculation. F. Survival rates of the transgenic plants inoculated with P. parasitica zoospores. The rates were measured at 4 dpi. The experiments were repeated four times in all transgenic lines with similar results. Twenty plants were used for each treatment in each experiment. Bars represent the standard deviation (SD). Different letters at the top of the columns indicate significant differences (P<0.01, Duncan's multiple range test).