Figure 4. Suppression of the H₂O₂ accumulation in N. benthamiana by PsCRN70.

A. DAB staining of the P. parasitica-inoculated N. benthamiana leaves. The H₂O₂ accumulation in the PsCRN70- and GFP- transgenic leaves were detected using DAB staining at 12 hpi. Photographs were taken after de-colorization of leaves with ethanol. B. The relative levels of DAB staining. The data were calculated by a combination of Photoshop and Quantity One for H₂O₂ accumulation in the indicated transgenic lines. The experiments were repeated three times in all transgenic lines with similar results. Four leaves were used for each treatment in each experiment. Bars represent the standard deviation (SD). Different letters at the top of the columns indicate significant differences (P<0.01, Duncan's multiple range test).