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. 2014 May 23;9(5):e98207. doi: 10.1371/journal.pone.0098207

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© 2014 Queiroz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A western blot of AMPKα, p-AMPKα (Thr 172), p70S6K and p-p70S6K (Thr 389) with β-actin as the control band. (B) Western blot ratio analysis of AMPK and p-AMPK (Thr 172), (C) p70S6K and p-p70S6K (Thr 389) in MCF-7 cells after treatment with metformin (10 mM) for 24, 48 and 72 hours. * p<0.05 vs. control; ** p<0.001 vs. control and *** p<0.0001 vs. control.