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. 2014 May 23;9(5):e98166. doi: 10.1371/journal.pone.0098166

Table 3. PCR programs used in this study.

GYP variants Denaturation Annealing Extension Cycles
GYP(A-B) 95°C 2 mins 60°C 2 mins 72°C 3 mins 35
GYP(A-B-A) 95°C 2 mins 60°C 2 mins 72°C 3 mins 35
GYP(B-A) 95°C 2 mins 58°C 2 mins 72°C 3 mins 35
GYP(B-A-B) 95°C 30 sec 58°C 30 sec 72°C 2 mins 30